What to do with larger proteins?
Larger proteins ( > 150 AA)
(1) Overcrowded spectra = too much information
(2) Fast relaxation = poor signal
How to fix?
(1) Technology: better electronics, bigger magnets, cold RF circuitry. Make magnets look like spaceships?
(2) Theory: Improved experiments ('TROSY' effect, non-linear data acquisition)
(3) Isotope labeling
see comparison of isotope properties
The first thing to do:
This experiment is similar to COSY and is called HSQC (heteronuclear quantum coherence spectroscopy)
Build a 3D-NOESY on top of that -> 3D NOESY-HSQC
a lot more to this story.... replace 12C with 13C, only label certain amino acids or certain sidechain groups ...
prev back to beginingCreated by Vlado Gelev, FBReagents.com